电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进肿瘤的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨宇宙射中央线是否是可通过转化生长因子-β（TGF-β）-介导的内膜-非典型转换 (EMT)来促进免疫蛋白的侵袭移至。使用增幅2Gy(60)Coγ中央线照射来源于人类生殖器官的6种免疫蛋白，记录与EMT相关的转变，这包括分别为了让光学仪器新科技，蛋白质斯塔夫基方法，免疫荧光新科技，划痕试验车和Transwell小室试验车来观察并检验蛋白组织其本质，EMT标示，侵袭移至灵活性等。采用酶联成免疫吸附法检验这些免疫蛋白中都TGF-β蛋白准确度，为了让特别酶抑制剂SB431542来评估TGF-β频谱通路在宇宙射中央线EMT中都的依赖性。经过增幅为2Gy照射的免疫蛋白中都假定间叶蛋白的强调，与所谓照射组相比其内膜标示减少，间叶蛋白标示增加，同时其侵袭转移灵活性减少，TGF-β蛋白准确度也减少。全面发现由A549宇宙射中央线诱导的EMT可通过对TGF-β频谱抑制发生关键时刻。这些分析表明TGF-β介导的EMT在宇宙射中央线诱导减少免疫蛋白侵袭转移灵活性中都起着关键依赖性。

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